Phyto-fabricated gold nanoparticles and heterologous platelet-rich fibrin potentiates cutaneous wound healing dynamics in canines.

This veterinary study investigated whether combining moringa leaf extract with gold nanoparticles could improve wound healing in dogs. Researchers treated 24 dogs with natural wounds using four different approaches: platelet-rich fibrin from other animals (h-PRF), gold nanoparticles combined with moringa leaf extract (AuNPs-MOE), both treatments together, or standard antibiotic dressing as a control. The team measured wound healing progress by examining the appearance of skin around wounds, fluid discharge, new tissue formation, and how much wounds contracted over time. They also tested blood samples and measured inflammatory proteins that indicate the body's healing response. The combination treatment group showed the most significant improvements in wound healing characteristics between days 3-7 after treatment began. This group had better skin color around wounds, reduced discharge, improved tissue formation, and greater wound contraction compared to other groups. Blood tests revealed that the combination treatment significantly reduced inflammatory protein IL-6 levels from day 0 to day 7, while platelet-rich fibrin alone was most effective at reducing another inflammatory protein called IL-1β. All treatments successfully reduced TNF-α, another inflammatory marker, suggesting they all had anti-inflammatory effects. The findings suggest that combining regenerative medicine approaches like platelet-rich fibrin with nanotechnology-enhanced plant extracts like moringa could offer superior alternatives to conventional wound treatment methods in veterinary medicine.